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Harvard University Research Assistant II in Cambridge, Massachusetts

51010BRAuto req ID:51010BRJob Code:403112 Research Assistant II Lab Location:USA - MA - Boston Business Title:Research Technician IISub-Unit:------------ Salary Grade:053Time Status:Full-time Union:55 - Hvd Union Cler & Tech Workers Basic Qualifications:At least 2 years of laboratory experience required. Demonstrated experience with molecular biology techniques, cell culture, and animals required. Must possess strong oral and written communication skills, effective and excellent interpersonalAdditional Qualifications:Bachelor’s degree preferred; preferably in a related discipline. Must possess a high level of initiative and investment in the success of research projects. Ability to maintain careful records of experiments; detail-oriented; meticulous work habits; possess strong organizational, analytical and communication skills; demonstrated multi-tasking and trouble-shooting skills; has the ability to work independently as well as part of a team.Additional Information:Offer contingent upon successful completion of a pre-placement medical evaluation.

Harvard offers an outstanding benefits package including:

Time Off: 3 - 4 weeks paid vacation, paid holiday break, 12 paid sick days, 11.5 paid holidays, and 3 paid personal days per year.

Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.

Retirement: University-funded retirement plan with full vesting after 3 years of service.

Tuition Assistance Program: Competitive tuition assistance program, $40 per class at the Harvard Extension School and discounted options through participating Harvard grad schools.

Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.

Wellness options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation, and complementary health services.

Harvard access to athletic facilities, libraries, campus events and many discounts throughout metro Boston.

The Harvard Medical School is not able to provide visa sponsorship for this position.

Department:ImmunologyPre-Employment Screening:Criminal, IdentitySchedule:​35 hrs. per week | Monday - Friday | 9:00 am - 5:00 pmJob Function:Research Job-Specific Responsibilities:The Sharpe laboratory at Harvard Medical School seeks a highly motivated individual to support research in immunology and gene targeting. This individual will independently perform and /or coordinate a wide range of highly sophisticated laboratory techniques, working closely with fellows and students to analyze the functions of immunoregulatory pathways. This includes immunologic techniques (T cell purification from mice, assays of T cell function, cytokine ELISA assays, multiparameter flow cytometry), molecular biology procedures such as PCR, and RNA and/or DNA preparation from tissues), and basic microscopy (cutting sections, staining slides, and imaging slides with a microscope). Animal work is required (measuring tumors, weighing mice, survival bleeding, i.p. and s.c. injection), mouse husbandry (setting up breeders, genotyping progeny). S/he will analyze and summarize data, and organize into lab notebooks, and report experimental results weekly. This individual may independently troubleshoot and optimize techniques. This individual also will assist with lab organization, maintenance of lab equipment, and lab safety activities.

This individual will also assist with cell culture aspects of gene targeting in mouse embryonic stem (ES) cells, a critical step for generating knock-out mice. This involves 1) culturing and performing gene targeting in specialized ES cells; 2) maintaining and quality controlling ES cell stocks; 3) preparing DNA from targeted ES cells, and 4) expanding targeted ES cells for microinjection into blastocysts. In addition, this individual will interact with investigators on gene targeting projects, maintain careful records of gene targeting experiments, and maintain equipment and supplies needed for ES cell culture work.School/Unit:Harvard Medical School EEO Statement:We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.