Beth Israel Deaconess Medical Center Research Assistant II in Boston, Massachusetts
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Research Assistant II
Department Description:The Boston Nutrition and Obesity Research Center (BNORC) Functional Genomics and Bioinformatics core is housed at BIDMC within the labs of Evan Rosen (http://evanrosenlab.com) and Linus Tsai (www.tsailab.com). The purpose of this core is to provide next generation sequencing services to investigators within BNORC and the Boston area looking to expand their research capabilities. We utilize cutting-edge next generation technologies and sequencing platforms to better understand the genetic, epigenetic, and tissue/cell type bases of metabolic disease. In particular, the Rosen/Tsai labs study the biological circuits and gene regulatory networks underlying obesity and type 2 Diabetes in human and rodent models. Our labs and core provide a vibrant research environment with close links to top academic institutions and hospitals across the Boston area and provides the potential for contributions to be used and recognized worldwide. We are currently seeking a talented, motivated research assistant with a background in next generation sequencing library construction to join our team to perform ChIP-seq, RNA-seq, ATAC-seq, and single cell/nucleus RNA-seq experiments.
The individual will work under guidance of Drs. Rosen and Tsai to conduct bench level experiments to support 1) ongoing core projects from BNORC clients (RNA-seq, ChIP-seq, ATAC-seq) and 2) development and execution of ongoing single cell RNA-seq production work in adipose tissue from mouse and human samples.
• Perform nucleic acid extractions and amplifications from clinical and research samples.
• Perform standard molecular biological techniques, including PCR, RT-PCR
• RNA-seq, ChIP-seq, ATAC-seq, CUT&RUN/CUT&Tag NGS library construction
• Generation of single cell and single nucleus libraries by Drop-seq/10X genomics platforms
• Operate and maintain laboratory equipment
• Prepare, order, and maintain stocks of necessary reagents, solutions and supplies
• Record experimental designs, results, and conclusions in both electronic and paper format. Prepare summary reports.
• Troubleshoot as necessary and identify areas for improvement.
• Consult with other senior scientists or scientific literature as needed. Maintain contact with target vendors for new products, applications, or product issues.
Job Location:Boston, MA
Job Summary:Under general supervision of the principal investigator, conducts a variety of routine and specialized laboratory tests using molecular, cell biology and/or biochemistry techniques. Performs lab procedures with speed, precision, and understanding of theory behind tests and procedures. Performs Level I responsibilities with a higher level of problem resolution, analytical ability, and self-direction.
Performs routine and non-routine laboratory tests which may include DNA sequencing, gene cloning, in situ hybridization, immunohistochemistry, northern blotting; synthesizes and interprets results.
May operate and maintain moderate to complex lab equipment such as spectrophotometer, flow cytometer, etc.
Using analytical abilities and sound judgment, makes decisions regarding the reliability and accuracy of results, repeating procedures when necessary.
May assist with planning specific research procedures and coordinating schedules of tests and procedures
May prepare written and/or verbal reports on status of research and the technical procedures used, including statistical and graphic summaries of findings and possible conclusions.
Bachelor's degree in Life Sciences or related scientific field required.
1-3 years related work experience required.
Bachelor's degree and one year of experience or a Masters degree with requisite knowledge and skills.
Experience with computer systems required, including web based applications and some Microsoft Office applications which may include Outlook, Word, Excel, PowerPoint or Access.
Decision Making:Ability to make decisions that are based on specific instructions, standard practices and established procedures which generally require little or no supervision.
Problem Solving:Ability to address problems that are routine, somewhat repetitive and generally solved by following clear directions and procedures and by identifying opportunities for process improvements.
Independence of Action:Ability to follow general instructions and procedures as provided. Work is monitored by supervisor/manager.
Written Communications:Ability to read, and write in English in order to understand basic safety instructions and take direction from supervisors; communicate effectively with patients, families and other medical center staff; and respond to basic questions.
Oral Communications:Ability to understand spoken English in order to follow basic safety instructions and take direction from supervisors; communicate effectively in basic English with patients, families and medical center staff in response to routine questions.
Knowledge:Ability to demonstrate basic knowledge of fundamental concepts, practices and procedures with the ability to use them in routine situations. Specialized knowledge not required.
Team Work:Ability to interact respectfully with other employees, professional staff and/or external contacts to offer ideas, identify issues, obtain information or deliver services.
Customer Service:Ability to demonstrate a positive attitude and respond to requests in a timely and respectful manner.
Physical Nature of the Job:
Light work: Exerting up to 20 pounds of force frequently to move objects. Some elements of the job are sedentary, but the employee will be required to stand for periods of time or move through out the hospital campus